Hello
As Zozo_mp told you in point n°1, you have to define your imposed movements (the static analyses of your part file do not have any). Finite element analysis considers your part as a set of points connected by springs. This is the network. To oversimplify, think of your test tube as one large spring. To study how it behaves, you can:
- Either fix one end and pull the other over a certain distance, measuring the effort to do this;
- Either fix one end and put an effort on the other side by measuring the resulting increase in distance
In the same way in the software, you must either block your test tube on one side and move the other side, or block one side by putting an effort on the other side. I personally advise you to simplify your problem first, then to make it more complex as you learn the tool (this was the meaning of my previous post).
In particular, I advise you first to learn how to put an imposed displacement (orange on the image) and an effort (easier to find a valid value like this; in purple on the image) on the two extreme sides:
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Afterwards, you can try to apply your boundary conditions (imposed displacements & forces) on your virtual faces that you have defined with the separation lines (it's already not bad if you know how to do that):
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Finally, you can tackle the more detailed models offered by Zozo_mp (elastic support, analysis within the specimen+jaw assembly, etc.) which will not lack interest for your training/knowledge. This will teach you to take a step back from what you are doing.
Don't hesitate to do a simulation by modeling, which will allow you to compare the results after the fact :
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In the two simplified cases that I have proposed, I have been able to see that the stress in the fillet area of the specimen is almost identical in both cases (6.581 MPa & 6.575 MPa). If you try, however, you will see that the max stress increases, at the separation line in model 2, which does not seem to me to be very realistic.
PS: a good way to train is to use the tutorials integrated into solidworks by going to the menu "?/SOLIDWORS Simulation/Tutorials ":
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Good luck
M.